Biomolecules are essential, large polymeric compounds produced by living cells that play crucial roles in the structure and processes of living organisms. They encompass proteins, peptides, polynucleotides, carbohydrates, lipids, vitamins, and coenzymes. Due to the complexity of these large molecules, their diverse chemical nature, and the need to consider biological activity, an effective characterization technique is necessary. Among the various available separation and analysis methods, high-performance liquid chromatography (HPLC) is the most commonly utilized.